| 論文作者 |
Li, R; Li, Y; Jiang, K; Zhang, LJ; Li, T; Zhao, AH; Zhang, Z; Xia, YL; Ge, K; Chen, YQ; Wang, CN; Tang, WT; Liu, SN; Lin, XX; Song, YQ; Mei, J; Xiao, C; Wang, AX; Zou, YJ; Li, X; Chen, XJ; Ju, ZY; Jia, W; Loscalzo, J; Sun, Y; Fang, W; Yang, Y; Zhao, YZ |
| 摘要 |
Arginine is one of the most metabolically versatile amino acids and plays pivotal roles in diverse biologicaland pathological processes; however, sensitive tracking of arginine dynamicsin situremains technicallychallenging. Here, we engineer high-performance fluorescent biosensors, denoted sensitive to arginine(STAR), to illuminate arginine metabolism in cells, mice, and clinical samples. Utilizing STAR, we demonstratethe effects of different amino acids in regulating intra- and extracellular arginine levels. STAR enabled live-cellmonitoring of arginine fluctuations during macrophage activation, phagocytosis, efferocytosis, and senes-cence and revealed cellular senescence depending on arginine availability. Moreover, a simple and fast assaybased on STAR revealed that serum arginine levels tended to increase with age, and the elevated serum argi-nine level is a potential indicator for discriminating the progression and severity of vitiligo. Collectively, ourstudy provides important insights into the metabolic and functional roles of arginine, as well as its potentialin diagnostic and therapeutic applications. |
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